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Image Search Results
Journal: Hepatology Communications
Article Title: Sirtuin3 promotes the degradation of hepatic Z alpha-1 antitrypsin through lipophagy
doi: 10.1097/HC9.0000000000000370
Figure Lengend Snippet: Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by Nephelometry. Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Article Snippet: The diagnosis of individuals with AATD was based on AAT genotyping and AAT serum level measurement using
Techniques: Microscopy, Staining, Clinical Proteomics, MANN-WHITNEY, Western Blot, Expressing, Control, Immunohistochemistry, Real-time Polymerase Chain Reaction