nephelometry dade behring diagnostic Search Results


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Behring Diagnostics aat serum level measurement using nephelometry
Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Rate Nephelometry, supplied by Behring Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Nephelometry Instrument, supplied by Behring Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Hs C Reactive Protein (Crp) Malvern, Pa (Dade Behring) Nephelometry, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Nephelometry, supplied by Dade Behring, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
C3c Nephelometry, supplied by Behring Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Nephelometry System, supplied by Behring Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by <t>Nephelometry.</t> Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
Nephelometry Kit, supplied by Behring Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by Nephelometry. Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

Journal: Hepatology Communications

Article Title: Sirtuin3 promotes the degradation of hepatic Z alpha-1 antitrypsin through lipophagy

doi: 10.1097/HC9.0000000000000370

Figure Lengend Snippet: Characterization of the humanized MAAT and ZAAT mouse model. (A) Representative IHC microscopy at ×10 of PAS-D staining of liver tissue collected from Pi*M and Pi*Z mice performed by the University of Florida Pathology core. The focused panel is at ×20. The arrow indicates the PAS-D–positive AAT inclusion bodies (n=3). (B) Plasma human AAT levels measured by Nephelometry. Statistical analysis by Mann-Whitney U test (n=6). (C) Western blotting depicting differential protein expression of Sirt3 and intracellular human AAT with Gapdh as a loading control between Pi*M and Pi*Z mouse livers. (D) Total Sirt3 densitometry normalized to Gapdh between Pi*M and Pi*Z mice. Statistical test by unpaired t test (n=3). (E) Linear regression analysis of mitochondria Sirt3 fraction (28 kDa) density and hAAT density (n=3). (F) Representative IHC staining microscopy images at ×20 and ×40 of Sirt3 in Pi*M and Pi*Z liver tissues performed by the University of Florida Pathology core (n=3). (G) qPCR data showing Sirt3 expression differences between Pi*M and Pi*Z liver tissues normalized to 18S housekeeping gene. Data displayed as fold changes compared with control samples. Statistical analysis by Mann-Whitney U test (n=3). Abbreviations: AAT, alpha-1 antitrypsin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; n.s., nonsignificant; qPCR: quantitative polymerase chain reaction; Sirt3, mouse Sirtuin3. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: The diagnosis of individuals with AATD was based on AAT genotyping and AAT serum level measurement using nephelometry (Behring Diagnostics).

Techniques: Microscopy, Staining, Clinical Proteomics, MANN-WHITNEY, Western Blot, Expressing, Control, Immunohistochemistry, Real-time Polymerase Chain Reaction